WBC manual count using hemocytometer
*Introduction
*Material
Blood sample (EDTA anticoagulated blood or capillary blood) WBCs diluting pipette Diluting solution Hemocytometer microscope*
The Hemacytometer
contains 2 Neubauer counting chamber Each chamber contains: *4 WBC counting squares *Each contains 16 squares*
Methodology
draw blood up to 0.5 marks on WBCs pipette and complete to 11 with WBCs diluting solution(Turk‘s solution).Mix for 2-3 minute. *Turk's solution
*
Charge hemacytometer
Discard the first 4-5 drops Place tip of the pipette at the edge central platform Then let the hemacytometer to stand on the bench for 3-5 minute.*
Count and calculate:
*Turk's solution
*Calculation
*Calculation
The dilution factor= total volume/sample volume = 11-1/0.5 =20*
Calculation
counted volume = The total volume of the 1 large square= = (width x length x depth ) = (1mm x 1mm x 1/10 mm) =1/10
*
Calculation
*Example:
*Normal values of WBCs:
*Significance of the test
Leukocytosis Occur as an indicator of body defense against foreign materials (bacteria, parasites, toxins) Metabolic disorders Chemical and drug poisoning Acute hemorrhage*
Significance of the test
Leukopenia Result from X-ray therapy Alcoholism Antibiotic therapy Typhoid infection Measls Infectious hepatitis TB And cirrhosis of the liver*
THANKS
Thanks
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