Microbiology-P-2 D. Esraa D. T.
1
Helicobacter pylori
Microscopical appearance:
G- curved or slightly spiral bacterium ,Motile, non sporeforming , non capsulated
Microaerophilic (require 5% co2)
Grow on selective medium (skirrows medium) contains antibiotics for selectivity
♠ Vancomycin for inhibition of G+ bacteria
♠ Colistin for inhibition of G- bacteria
♠ Amphotericin for inhibition of fungus.
Colonies are 1-2 mm in diameter , translucent
Microbiology-P-2 D. Esraa D. T.
2
Biochemical tests
Urease +ve
Oxidase & catalase +ve
Specimens:
biopsy from stomach (by endoscopy)for isolation of this bacterium ,serum
(detection antibidies),stool(detection of antigen) for detection of this bacterium.
Culture:
On Skirrows medium for 3-5 days
Confirmative test
Gram stain
Oxidase
Urease