Helicobacter pylori

Microscopical appearance

G- curved or slightly spiral bacterium Motile, non sporeforming , non capsulated

Culture characterstic

Microaerophilic (require 5% co2)Grow on selective medium (skirrows medium) contains antibiotics for selectivity ♠ Vancomycin for inhibition of G+ bacteria ♠ Colistin for inhibition of G- bacteria♠ Amphotericin for inhibition of fungus.Colonies are 1-2 mm in diameter , translucent skirrow media

Biochemical tests

Urease +ve Oxidase & catalase +ve

Diagnostic laboratory tests

Specimens: biopsy from stomach (by endoscopy)for isolation of this bacterium ,serum (detection antibidies),stool(detection of antigen) for detection of this bacterium. Culture: On Skirrows medium for 3-5 days Confirmative test Gram stain Oxidase Urease